Heterogeneous distribution of enzymes among plasma-membrane fragments sedimenting with the microsomal fraction of rat liver
نویسندگان
چکیده
منابع مشابه
Heterogeneous Distribution of Enzymes in Submicrosomal Membrane Fragments
Microsomal membranes are postulated to contain either a homogeneous arrangement of individual enzymes or groupings of functionally related enzymes. In the present study we attempt to distinguish between these hypotheses in subfractions of rough microsomes from rat liver. After sonication, the individual vesicles that make up the rough-membrane fraction average less than 1/100 of their previous ...
متن کاملRemoval of plasma-membrane fragments from rat liver microsomal preparations by agarose-bound concanavalin A
The membrane fraction of a rat liver post-mitochondria1 supernatant is largely derived from endoplasmic reticulum, but also contains plasma-membrane fragments and Golgi membranes (see, e.g., Depierre & Dallner, 1975). The subfractionation of microsomal preparations normally depends on the use of high centrifugal fields, and hence large hydrostatic pressures. We have attempted to avoid these har...
متن کاملA rapidly sedimenting fraction of rat liver endoplasmic reticulum.
Balance-sheet experiments carried out to account for the distribution of endoplasmic reticulum fragments during subcellular fractionation of rat liver showed that a large proportion of these fragments are present in the pellets of low-speed centrifugation. Using glucose-6phosphatase and RNA as markers we found that approximately 50 % of the fragments of endoplasmic reticulum sedimented in the p...
متن کاملMetabolism of benzo(e)pyrene by rat liver microsomal enzymes.
Metabolites of benzo(e)pyrene (B[e]P) formed upon incubation of [3H]-B[e]P with hepatic microsomes from control and induced rats have been separated by high-pressure liquid chromatography and identified by comparison of retention times, absorbance and fluorescence spectra with those of synthetic standards. The major metabolite produced was B[e]P-4,5-dihydrodiol, accounting for 20-30% of the tot...
متن کاملthe effect of using visual aids on the development of speech act of disagreement among iranian intermediate efl learners
abstract the present study tried to investigate the effect of visual aids (films) on the development of the speech act of disagreement among iranian efl intermediate learners. to this end, the researcher selected 40 homogeneous intermediate learners based on their scores on oxford placement test. .the subjects then divided into control group and experimental group. both classes were tested by ...
ذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1974
ISSN: 0264-6021
DOI: 10.1042/bj1420667